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HATODAS

Heavy-atom Database System (HATODAS) is a WWW-based tool designed to assist the heavy-atom derivatization of proteins. The conventional procedure for the preparation of derivatives is usually a time-consuming `trial-and-error' process. The present program provides a solution for this problem using a database of known heavy-atom derivatives. A database search suggests potential heavy-atom reagents for any target protein based on its amino-acid sequence and crystallization conditions. A mining of the database identified 93 preferred motifs for heavy-atom binding. The motifs are observed frequently at the actual heavy-atom-binding sites encountered in the process of structure determination.

 

Heavy-atom Database System: a tool for the preparation of heavy-atom derivatives of protein crystals based on amino-acid sequence and crystallization conditions.  Acta Crystallographica Section D61, 1302-1305, (2005).

HATODAS II - heavy-atom database system with potentiality scoring.  Journal of Applied Crystallography, 42, 540-544. (2009).

    

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Protein Crystallography Research Group,

RIKEN SPring-8 Center

1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan

E-mail : Michihiro Sugahara